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Humblefish

Hybrid TTM to treat all parasites!

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Humblefish

*** The information contained here is only EXPERIMENTAL at the moment. Use at your own risk!!! ***

 

So, I wanted to start a discussing regarding the use of Hydrogen Peroxide (H2O2) in conjunction with Tank Transfer Method. For those who may not be familiar with TTM read this: Tank Transfer Method

 

TTM is an effective (non-chemical) treatment for Marine Ich; however the weakness has always been its inability to eradicate other external parasites/worms such as Velvet, brook, uronema, flukes and turbellarians. What if combining TTM with H2O2 could widen the spectrum of treatment?? 

 

In this field trial, a single treatment with 75 mg/L hydrogen peroxide greatly reduced levels of Amyloodinium (velvet) infestation, and a second treatment 6 days later reduced Amyloodinium trophonts to a nondetectable level: The Application of Hydrogen Peroxide as a Treatment for the Ectoparasite Amyloodinium ocellatum (Brown 1931) on the Pacific Threadfin Polydactylus sexfilis

 

IME; any chemical (e.g. formalin, acriflavine) capable of removing Velvet trophonts right on a fish is also capable of killing "surface" parasites & worms such as brook, uronema, flukes and turbellarians (Black Ich). So I am theorizing that a "Hybrid TTM" using H2O2 could eliminate most external parasites/worms by following this protocol:

 

Day 1 – Fish is placed in initial QT. 30 minutes before transfer (Day 4), turn off all pumps/water movement and dose 150 ppm H2O2 as outlined here: Hydrogen Peroxide

 

Day 4 – Roughly 72 hours later transfer the fish to new tank. The time of day you do the transfer is unimportant, but never exceed 72 hours from the last transfer. The temperature and SG of the new tank should match the old one perfectly, so you can just catch & release (no acclimation). Transfer as little water as possible with the fish.

 

Day 7 – Repeat. 30 minutes before transfer (Day 10), turn off all pumps/water movement and dose 150 ppm H2O2 as outlined here: Hydrogen Peroxide

 

Day 10 – Repeat.

 

Day 13  Repeat and done (fish should now be parasite & worm free!!!)

 

^^ Please note that H2O2 is only dosed twice, 6 days apart and for a maximum of 30 minutes. This is to ensure all velvet trophonts have been eliminated and also lines up for worms that need to be treated a second time due to hatchlings. Everything else about doing TTM stays exactly the same!

 

Will this work?? I honestly don't know. The science behind it is sound and everything lines up, but it needs to be thoroughly tested before going mainstream. Since starting my business, I'm finding myself with less & less time to experiment so I'm hoping some of you will pick up my slack. 

 

Why TTM is still needed with H2O2: IMO; it is unlikely that H2O2 can penetrate through a fish's epithelium (outer skin layer) to reach Ich trophonts. Eliminating "surface" parasites & worms is a more realistic expectation. So, TTM is still needed to prophylactically address the possibility of Ich!

 

Other thoughts/concerns:

  1. Avoid using H2O2 on fish with an open wound or obvious infection. Although H2O2 is an antiseptic, it has been found to slow the healing process and possibly worsen scarring by killing the healthy cells surrounding a cut.
  2. It is only reasonable to assume that H2O2 treats parasites/worms on the outside of a fish. Internal parasites & worms will need to be treated separately: Internal Issues
  3. Post treatment, it is always wise to observe the fish in a non-medicated observation tank. This applies whether you are utilizing Traditional TTM or Hybrid TTM, to ensure treatment was successful. Using black mollies in this observation tank can help ensure no diseases are still present: Black Molly Quarantine
Edited by Humblefish
Dosage increased to 150 ppm

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Humblefish

^^ Just wanted to point out that there's no reason why 45 mins in formalin couldn't be used in lieu of H2O2: Formalin (use the bath treatment dosage)

 

45 minutes in formalin has the same killing effect on surface parasites/worms, and you can continue to run filtration/pumps with it in the water. However, it must be proper formalin (37% formaldehyde) for consistency. The main drawback to using formalin is it is a known carcinogen.

 

Everything stays the same as above, just do a 45 min formalin bath instead of a 30 min H2O2 bath prior to transferring. (I know many hobbyists already doing this with success.)

 

Further reading on using formalin to control fish parasites: http://fisheries.tamu.edu/files/2013/09/Use-of-Formalin-to-Control-Fish-Parasites.pdf

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Tamberav

Very interesting...thank you. I will try this if I get a fish that comes with obvious velvet or brook. That thankfully doesn't seem to happen too often.

 

Honestly this would be a breakthrough for Canadians if it works since they don't have access to normal medications.

 

@Clown79 😮

 

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Tamberav

@Humblefish when you say business you are talking about Humblefish Aquatics? Any plans to get the website up soon? 

 

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NaturallyKait
3 hours ago, Tamberav said:

Very interesting...thank you. I will try this if I get a fish that comes with obvious velvet or brook. That thankfully doesn't seem to happen too often.

 

Honestly this would be a breakthrough for Canadians if it works since they don't have access to normal medications.

 

@Clown79 😮

 

 

 

That’s exactly what I was thinking, with us having no access to real meds up here except for anything we happen to have stashed away before the ban this could be hugely helpful. 

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Melfy77
1 hour ago, NaturallyKait said:

That’s exactly what I was thinking, with us having no access to real meds up here except for anything we happen to have stashed away before the ban this could be hugely helpful. 

Following for sure!!! I'm also Canadian. After a velvet wipeout, watching all my fish die because I had nothing to save them, was not a nice experience. Pretty much felt like a fish murderer.

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NaturallyKait
Just now, Melfy77 said:

Following for sure!!! I'm also Canadian. After a velvet wipeout, watching all my fish die because I had nothing to save them, was not a nice experience. Pretty much felt like a fish murderer.

It’s so unfair. No vets here will prescribe for fish so all we can do is watch them die. I have a few meds coming that a friend of mine happened to stash away before the ban and was willing to share, but they don’t cover everything. I have no copper so that’s a big set back. 

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Clown79
7 hours ago, Tamberav said:

Very interesting...thank you. I will try this if I get a fish that comes with obvious velvet or brook. That thankfully doesn't seem to happen too often.

 

Honestly this would be a breakthrough for Canadians if it works since they don't have access to normal medications.

 

@Clown79 😮

 

 

 

Thank you!

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Humblefish

Recommended dosage for the 30 min H2O2 bath is now 150 ppm. Dosing instructions have been updated in the OP.

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