hypostatic Posted November 26, 2014 Share Posted November 26, 2014 Have you seen this? http://www.hhmi.org/news/new-microscope-collects-dynamic-images-molecules-animate-life White blood cell attacking other cell: http://vimeo.com/109402660 http://www.vimeo.com/album/3098015 I think this is my favorite video of the bunch. The microscope is just so freaking fast: http://vimeo.com/109403880 Link to comment
Mr. Microscope Posted December 15, 2014 Author Share Posted December 15, 2014 Hello All,As some of you know, I lost my sea urchin during a transition back at the end of August. I was finally able to put forth a fitting memorial to it in the form of electron microscopy. Here it was in all it's reefy glory.Echinometra ViridisIn the above pic, you can see little lines in the spines. Here they are up close.I also looked at the spine in cross section.Check out how the bone is built up with a face-centered hexagonal pattern.Additionally, I took a look at the body shell. It is composed of many nodules where the spines connected to the body and could rotate freely like a knee or elbow joint.Holes lined the shell where podia would protrude.The resolution here really doesn't do the pic justice. Here is a link to download an ultra-high resolution .tif of this image if you're interested. It should look pretty awesome on one of those new screens. Resolution is 5120 x 3560. The download is about 20MB.https://dl.dropboxusercontent.com/u/6760404/Shell%2002%20UHD.tifFinally, here is a video I made a while back featuring this urchin. Link to comment
Dryland Posted December 15, 2014 Share Posted December 15, 2014 Dude, this thread is so freaking cool. Link to comment
Chris! Posted December 16, 2014 Share Posted December 16, 2014 That is pretty sweet!!!!! Link to comment
Mr. Microscope Posted February 3, 2015 Author Share Posted February 3, 2015 Dude, this thread is so freaking cool.Thank you Dryland! I don't get to update it nearly as frequently as I'd like. That is pretty sweet!!!!!Thank you Chris! Link to comment
Felicia Posted February 3, 2015 Share Posted February 3, 2015 Love this! Glad Ben shared this over on my thread Link to comment
Mr. Microscope Posted February 3, 2015 Author Share Posted February 3, 2015 Thank you Felicia! They're really neat animals. Link to comment
Mr. Microscope Posted February 9, 2015 Author Share Posted February 9, 2015 Awesome as always!Thank you Pico! Link to comment
AllOutAfrican Posted March 11, 2015 Share Posted March 11, 2015 wow this thread is so interesting its amazing to see a coral so close up thanks for taking the time to do this.. i havent had time to read through it all but will defiantly read through.. Link to comment
Mr. Microscope Posted March 11, 2015 Author Share Posted March 11, 2015 wow this thread is so interesting its amazing to see a coral so close up thanks for taking the time to do this.. i havent had time to read through it all but will defiantly read through.. Thank you AllOut! I'm actually about to update it again. Stay tuned. Link to comment
Mr. Microscope Posted March 11, 2015 Author Share Posted March 11, 2015 Hello All!Many of you may have seen Charlie Mazel's MACNA speech. If not, here it is:https://youtu.be/3rC4G84OWIgCharlie is the guy behind the NightSea fluorescence viewing system. He's pretty much responsible for the new passion for so-called, fluorescence night diving, and also markets systems to adapt pretty much any stereo microscope for fluorescence. Yesterday, I had the good fortune to have Charlie come to my lab and do a demo of his fluorescence systems on one of my microscopes.(No, we did not plan to coordinate outfits. lol )Here's our microscope retrofitted with the NightSea system. Here's Dr. Mazel explaining the system: https://youtu.be/ucPX4qsQQww One of my bubble tip anemones recently split, but it did so somewhat unevenly. I was left behind with one of the smallest clones I've ever seen. It's no larger than a quarter. So, I figured it would be a great candidate to image with this system. I carefully took it out of the basket in my tank and brought it to my facility for the day. Here's a picture of the mother before it split. This was taken under an estimated 12-14K. There are several lampsets/filters available for the system and Charlie brought the royal blue lamp to stimulate green fluorescence, a cyan lamp for yellow-orange fluorescence, and a green lamp for red fluorescence. I managed to image the BTA under all three conditions. Please forgive any blurriness as I was only able to get pictures by holding my Canon point-and-shoot camera up to one of the oculars of the microscope. From left to right: RB, Cyan, and Green source. Here's the lamp heads and corresponding filters starting with UV, RB, Cyan, and Green.The filters (Yellow, orange, and red) cut out signal from the LED that would obscure the image. Unlike taking pictures of our corals under blue LED's and having the camera blown out by blue, the filter removes the blue so that we are only left with the true fluorescent signal. The figure below shows the effect of filtering with the cyan source. The image on the left is unfiltered. You can clearly see the the effects of fluorescence as the cyan source excites the proteins of the anemone, but the picture is obscured by the cyan background and effects of cyan light on the fluorescent signal. The image on the left has been filtered. Notice, we still see the fluorescent signal from the BTA, but the background is completely devoid of cyan. It was amazing how the green fluorescent proteins (GFP) lit up. This BTA was of the speckled variety. Here, we get to see the intense emissions from these speckles made up of cells saturated with GFP. This image was taken utilizing the green LED source to excite red fluorescent proteins (RFP). Looking closer with the royal blue emission, we were able to see how the orange and yellow appearance to the tentacles is actually composed not of orange or yellow pigments, but of a coalescing of GFP and RFP in a graded series of concentrations. Using Photoshop, I isolated the green and red signals so you can observe their overlap to create the rainbow effect we see in our "Rainbow Bubble Tips Anemones." Here, closer to the base of the tentacle, you see an individual cell lit up with GFP. To exit, just one more shot of the BTA's under all three conditions. These pics were not taken under the microscope, but with viewing shield filter I placed in front of my camera. Click to view larger. I had a great time with Charlie and I hope that our facility will be purchasing one or two of these systems in the near future. This tool will definitely be useful for projects from biology and cancer research, to hard and soft materials science and engineering. I was truly inspired by this experience. I'm ready to go find a huge yellow screen to put in front of my tank for night viewing. lol Link to comment
Slowtwitch Posted March 11, 2015 Share Posted March 11, 2015 Very cool write up. Thanks for sharing! Link to comment
Maniu Posted March 11, 2015 Share Posted March 11, 2015 Love this thread and all the work you have done. Awesome Link to comment
neuwave Posted March 11, 2015 Share Posted March 11, 2015 Yep. This made my day. Awesome blend of photography and science! Link to comment
Mr. Microscope Posted March 12, 2015 Author Share Posted March 12, 2015 Very cool write up. Thanks for sharing!Thank you for checking it out Slow! Love this thread and all the work you have done. AwesomeThank you Maniu! It's a lot of fun! Yep. This made my day. Awesome blend of photography and science!Awesome! Thank you neuwave! Link to comment
MC5858 Posted March 12, 2015 Share Posted March 12, 2015 I'm going to try some micro CT X-ray, I think I can get down to .35 microns. It's good to be a geek. Link to comment
Mr. Microscope Posted March 12, 2015 Author Share Posted March 12, 2015 I'm going to try some micro CT X-ray, I think I can get down to .35 microns. It's good to be a geek.Cool cool! Pretty stellar resolution on that. What are you imaging? Link to comment
MC5858 Posted March 12, 2015 Share Posted March 12, 2015 Last week it was mice femur's for bone density. Not my main gig but entertaining. Link to comment
Mr. Microscope Posted March 12, 2015 Author Share Posted March 12, 2015 Last week it was mice femur's for bone density. Not my main gig but entertaining.Neat! What is your main project? Link to comment
MC5858 Posted March 12, 2015 Share Posted March 12, 2015 I'm an engineer mostly installing Nuclear Magnetic Resonance Spectrometers. Occasionally I get to play with something interesting at the universities I visit. And lest I forget I'd like to compliment you on your imagery. I believe I caught some of Charlie's speech on youtube and plan on trying some filters to get the blue out of my pictures. Link to comment
Polarcollision Posted March 12, 2015 Share Posted March 12, 2015 :eek: :eek: :bowdown: :bowdown: That is all Link to comment
smiz Posted March 12, 2015 Share Posted March 12, 2015 I'm an engineer mostly installing Nuclear Magnetic Resonance Spectrometers. Google confirmed, these are real words Link to comment
Mr. Microscope Posted March 12, 2015 Author Share Posted March 12, 2015 I'm an engineer mostly installing Nuclear Magnetic Resonance Spectrometers. Occasionally I get to play with something interesting at the universities I visit. And lest I forget I'd like to compliment you on your imagery. I believe I caught some of Charlie's speech on youtube and plan on trying some filters to get the blue out of my pictures. Cool! We have a couple of those here at Northwestern. I can actually see them from my office. Yeah, I usually just use a white balance, but this has me rethinking things. :eek: :eek: :bowdown: :bowdown: That is all Thank you PC! Haha! I was just trying to reply here and look what popped up! I guess 10 is the max. Google confirmed, these are real wordsLOL! Link to comment
Polarcollision Posted March 12, 2015 Share Posted March 12, 2015 Cool! We have a couple of those here at Northwestern. I can actually see them from my office. Yeah, I usually just use a white balance, but this has me rethinking things. Thank you PC! Haha! I was just trying to reply here and look what popped up! I guess 10 is the max. LOL! Yes it it, I tried to put a whole row of each! Phtttbt. limiting our praise, ;-) Link to comment
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