wankreas Posted September 14, 2011 Share Posted September 14, 2011 Check it out! I made it into Advanced Aquarist! http://www.advancedaquarist.com/blog/scann...-of-pocillapora Congratulations!! /highfive Link to comment
mgargiullo Posted September 15, 2011 Share Posted September 15, 2011 These pics are fantastic! The chaeto is beautiful. Link to comment
TheUnfocusedOne Posted October 26, 2011 Share Posted October 26, 2011 So are your sputter coating your samples or are they able to withstand high mag? Generally curious, I usually work with polymers which melt at high magnifications (its really cool to watch). I would think stuff like the diatoms might melt or explode (seen stuff 'splode too). Link to comment
metrokat Posted October 26, 2011 Share Posted October 26, 2011 bump for more cool pics Link to comment
Mr. Microscope Posted October 26, 2011 Author Share Posted October 26, 2011 So are your sputter coating your samples or are they able to withstand high mag? Generally curious, I usually work with polymers which melt at high magnifications (its really cool to watch). I would think stuff like the diatoms might melt or explode (seen stuff 'splode too). For these samples I used an Osmuim coater because of all the nooks and crannies, but for normal SEM samples, yes. I sputter them with Au, Au+Pt, Pt+Pd, or something like that. I work with polymers too. They can be frustrating, especially in TEM. The beam practically shoots right through them if you forget to put in the Objective aperture. Even with, they sometime undergo considerable beam damage. bump for more cool pics I took some cool light microscopy shots of a feather duster the other day. I wanted to find an copepod to enter into the contest, but no luck. Gonna post those soon. I also had a branching monti RTN on me. So, I have that one ready for SEM imaging perhaps later this week if I have time. Link to comment
Deleted User 6 Posted October 26, 2011 Share Posted October 26, 2011 For these samples I used an Osmuim coater because of all the nooks and crannies, but for normal SEM samples, yes. I sputter them with Au, Au+Pt, Pt+Pd, or something like that. I work with polymers too. They can be frustrating, especially in TEM. The beam practically shoots right through them if you forget to put in the Objective aperture. Even with, they sometime undergo considerable beam damage. nerrrrrrd Link to comment
Mr. Microscope Posted October 26, 2011 Author Share Posted October 26, 2011 nerrrrrrd Link to comment
basser1 Posted October 26, 2011 Share Posted October 26, 2011 Way to go!! I'm for anyone from N-R getting recognition!! Link to comment
Mr. Microscope Posted October 28, 2011 Author Share Posted October 28, 2011 Light Microscopy of Feather Duster Worm Hello All! Well I put another piece of chaetomorpha under the microscope. This time I had intended to look for copepods to enter into the NR Image Contest. No luck. Instead, I happened upon this beautiful baby feather duster worm or, "Sabellidae" for those that want the scientific name. Enjoy! All this was not very easy to capture since this thing was wiggling around all over the place. For every in-focus image I took, there were probably ten that had blurry movement in them. Coming soo...SEM of Montipora Digitata. Stay tuned! Link to comment
Deleted User 6 Posted October 28, 2011 Share Posted October 28, 2011 pfft, i can do that with my iphone. SHOW ME SOMETHING REAL MR. MICROFACE. jk that's cool. also, i had one of those dusters once: Link to comment
Mr. Microscope Posted October 28, 2011 Author Share Posted October 28, 2011 pfft, i can do that with my iphone. SHOW ME SOMETHING REAL MR. MICROFACE. jk that's cool. also, i had one of those dusters once: Nice shot! Wow! Link to comment
Deleted User 6 Posted October 28, 2011 Share Posted October 28, 2011 nah, cooler seeing it way close up like yours. Link to comment
Mr. Microscope Posted October 30, 2011 Author Share Posted October 30, 2011 MOAR PICS! They're at the end of page 3. Link to comment
metrokat Posted October 30, 2011 Share Posted October 30, 2011 I saw them Mr. Microscope. I meant more. Link to comment
Mr. Microscope Posted October 31, 2011 Author Share Posted October 31, 2011 I saw them Mr. Microscope. I meant more. Ah..on the way! So cool! Thanks FICandy! Love the avatar pic! LOL Link to comment
Rabidgerbil38 Posted October 31, 2011 Share Posted October 31, 2011 Awesome stuff man, I just got to use a stereo light microscope for my Bio lab and it was awesome. Very trippy stuff when you can see magnified 3d Link to comment
Mr. Microscope Posted November 4, 2011 Author Share Posted November 4, 2011 Scanning Electron Microscopy of Montipora Digitata Hello All, As I mentioned before, I recently lost a digi frag to RTN. I had an extra couple minutes left over after looking at a sample for work. So, I grabbed a few images of this interesting SPS. Here is an image of the original frag. I was really sad to lose it since it had such amazing color. The polyps were bright red-orange and the flesh ranged from banana yellow to almost sky blue. The skeleton has some interesting structure: Here's where a polyp would come from: And the tip of one of those tines from the polyp area: I zoomed in a bit on the crystal structure of the skeleton (from about the middle of the above pic): Here's another area of the skeleton that I zoomed in on. There are some very different structures here: Check out these spherical features I found. ...who'd of guessed? Sample prep on this sample was similar to the pocillopora sample. Images were acquired on a Hitachi 4800 Field Emission Gun SEM. I'm thinking about perhaps doing oyster eggs or phyto next. Stay tuned! Link to comment
Ezsharkman Posted November 4, 2011 Share Posted November 4, 2011 i love this thread! its amazing to thing how infinitely big the universe is, and how its infinitely small too. my brain melts when i try to grasp that concept great shots Mr Micro! Link to comment
lakshwadeep Posted November 4, 2011 Share Posted November 4, 2011 Very cool! It's interesting how porous the structure is up close. You might consider viewing sponge spicules, which, like skeletons for SPS corals, are often the only way to distinguish species. Link to comment
Felicia Posted November 4, 2011 Share Posted November 4, 2011 I'm a graduate student in Materials Science and Engineering, so I'm pretty familiar with SEM and TEM, but I've never thought to stick coral in one. So cool. I used to work on biomimetic mineralization, so seeing the calcium carbonate crystal structure in these is awesome! Thanks for sharing! Are you a graduate student or a tech that works with the microscopes? Also, out of curiosity, what kind of samples do you normally look at? Link to comment
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