brandon429 Posted April 24, 2014 Share Posted April 24, 2014 thats a challenge tank man! are you going to salinity drop it Link to comment
jedimasterben Posted April 24, 2014 Share Posted April 24, 2014 Those pics are pre and post dip. A drop in salinity does nothing that won't kill everything else in the tank. These dinos have been able to survive a gradual change to ~60ppt. A rapid change is what is necessary. Link to comment
brandon429 Posted April 24, 2014 Share Posted April 24, 2014 but Im asking are you going to run that dip to kill em off, essentially a whole tank salinity treatment? dont blame you, thats decent coverage for sure. some of it looked like cyano to me Link to comment
jedimasterben Posted April 24, 2014 Share Posted April 24, 2014 Again, those pictures are pre and post dip. One dip was performed, more will come. Link to comment
brandon429 Posted April 24, 2014 Share Posted April 24, 2014 oh I see, its in the dying phase I get it now. well it will be neat to see how thorough it turns out Link to comment
jedimasterben Posted April 24, 2014 Share Posted April 24, 2014 That's not a dying phase. The dip was performed April 3, the last couple of pictures were from yesterday. Link to comment
Mr. Microscope Posted May 15, 2014 Author Share Posted May 15, 2014 Hello All, I finally got a couple minutes to look at the ostriopsys dinoflagellates under the EM. Here's the thing that was messing with my tank for a month or so. Removing the sand bed in combination with aggressive water changes and several 72 hour blackouts took care of it. Here's a final fairwell to this plague. To be honest, these were kinda boring and there were a lot more cool things in this sample. To start out, I pulled an isopod out of the algae. Also, there were a lot of cool diatoms in this sample. Check 'em out! Here's that one that's up front: This one is towards the back right of the first diatom image: Here's a football looking one: Nice and clean against some algae: Close up of this one: Link to comment
Felicia Posted May 15, 2014 Share Posted May 15, 2014 Thanks for sharing! That isopod is super creepy, but I like the diatoms Diatoms are always a ton of fun to look at under the microscope. Link to comment
Mr Pants Posted May 15, 2014 Share Posted May 15, 2014 Looks like you haven't managed to strip the membrane off the Ostreopsis. If you can remove the membrane you can see the thecal plates like here: Link to comment
Mr. Microscope Posted May 21, 2014 Author Share Posted May 21, 2014 Thanks for sharing! That isopod is super creepy, but I like the diatoms Diatoms are always a ton of fun to look at under the microscope.Thanks Felicia! It sure is and they sure are. lol Looks like you haven't managed to strip the membrane off the Ostreopsis. If you can remove the membrane you can see the thecal plates like here: Yup, I used Osmium as there was other stuff in there I wanted to preserve. It looks like it suffered some dehydration artifacts. Link to comment
Mr Pants Posted May 21, 2014 Share Posted May 21, 2014 Try skipping the Osmium and taking more ethanol dilution steps.. Link to comment
hypostatic Posted October 13, 2014 Share Posted October 13, 2014 Mr Microscope! Any more microscopy pics to share? PS - in a few weeks I'm gonna get to play with a stimulated emission depletion laser microscope for a bit Link to comment
Mr. Microscope Posted October 13, 2014 Author Share Posted October 13, 2014 Mr Microscope! Any more microscopy pics to share? PS - in a few weeks I'm gonna get to play with a stimulated emission depletion laser microscope for a bit Nothing at the moment. I might take a look at some sea urchin skeleton and spines sometime, but I've been too busy with actual research lately to throw anything reef related into the microscope for five minutes. Thanks for the bump! What will you be looking at in the STED? Very cool! Link to comment
picoreef78 Posted October 13, 2014 Share Posted October 13, 2014 Thanks for the pics. They are pretty sweet! Link to comment
hypostatic Posted October 13, 2014 Share Posted October 13, 2014 What will you be looking at in the STED? Very cool! SO much stuff lol. Worms, flies, yeast, stem cells, brain slices,and arabidopsis I think makes up most of it. Link to comment
Mr. Microscope Posted October 13, 2014 Author Share Posted October 13, 2014 Thanks for the pics. They are pretty sweet!Thanks pico! SO much stuff lol. Worms, flies, yeast, stem cells, brain slices,and arabidopsis I think makes up most of it. Fun fun! I'm working on a project with c. elegans right now. Done plenty of drosophila and brain too, but never looked at arabidopsis. Haven't really done any plant stuff since school. Link to comment
hypostatic Posted October 13, 2014 Share Posted October 13, 2014 ah worms have been my favorite model to work with so far. They dont call it the elegant model system for nothing Link to comment
hypostatic Posted October 21, 2014 Share Posted October 21, 2014 hey, I found this and I thought you'd like it: Link to comment
Mr. Microscope Posted October 21, 2014 Author Share Posted October 21, 2014 lol, silly FIB work. Link to comment
blizzardscout2 Posted October 28, 2014 Share Posted October 28, 2014 I have to say the pictures you have posted are amazing, it is pretty cool to gain perspective every once in awhile how small things are. Thanks. Link to comment
Mr. Microscope Posted October 28, 2014 Author Share Posted October 28, 2014 I have to say the pictures you have posted are amazing, it is pretty cool to gain perspective every once in awhile how small things are. Thanks. Thank you blizzard! I just saw an image taken under an EM of hydrogen atoms! It doesn't get much smaller than that. Link to comment
Mr. Microscope Posted October 29, 2014 Author Share Posted October 29, 2014 Thank you cj! Link to comment
East1 Posted October 30, 2014 Share Posted October 30, 2014 easily the coolest thread I've seen! Link to comment
Mr. Microscope Posted October 30, 2014 Author Share Posted October 30, 2014 Thank you East! Link to comment
Recommended Posts
Archived
This topic is now archived and is closed to further replies.