Can anyone tell me what the best settings are to take pictures of my nano cube with my nikon d60?

Are you taking pictures of fish or corals? Do you have a tripod?

Manual!

But seriously, what are you photographing? What kind of light? And what's your photo knowledge like?

You will get the best images from using the manual settings (with a few exceptions), given you know how to use them. If you don't it's definitely worth learning.

auto wo flash w tripod on raw if you're a beginner

manual wo flash w tripod on raw if you're not

no such thing as a best setting..Every tank and situation is different..what might be perfect for someone else..might not work at all for you.

Manual settings

Set a custom white balance

Put the camera on a tripod.

If you're shooting fish, you'll probably need a faster shutter speed. (Adjust aperture & ISO accordingly)
Corals you can slow it down a bit.

But 05X is right. Every situation is different.

For fish, I typically shoot with ISO 400, Shutter speed 1/25 or so and aperture as wide open as possible. The shutter speed will differ depending on how fast the fish is.

For corals, put the camera on a tripod, ISO 100 and aperture priority mode. Alter the aperture to suit what you need and let the camera determine how long the exposure time should be.

That's about as detailed I can get since your lighting will be different than what mine was.

Yeah I just want to shoot my corals. Ive got two 10,000k 50/50 bulbs in my nano cube and I do have a tripod. Anything else I can do for a better picture?

You can manually set the white balance, or you could edit it in post processing, but as far as the camera and it's settings are concerned, yeah that's about all I've got.

Here is a good read.

Melev's Photo Guide

Or this one:
Sticky at top of Photography Subforum

also, the best setting is 'M'.

I would suggest using a timer if you can, that will give you time to back away so the camera doesn't get jostled while it's exposing. For corals, if you set a low ISO you will need long exposures so you'll probably want to turn your pumps off.

Experiment with different settings and you'll find one you like. Every little adjustment can change the look of your images and sometimes you'll find you like something different than what others would suggest.

i just made a topic for my D50 same thing....what really made the difference is "pumps off" for me...